asymmetric PCR

Detection of Hepatitis B Virus Using Molecular Beacon and Asymmetric PCR

分子信标用于不对称PCR检测乙型肝炎病毒

Enhancing the hybridization efficiency of oligo microarray by asymmetric PCR

应用不对称PCR技术提高寡核苷酸基因芯片杂交效率

Results Preparation of ss probes by asymmetric PCR and single-pr imer PCR gets success .

结果不对称PCR及单引物PCR技术制备单链探针均获得成功；

Detection of dengue virus replicative intermediate and replicative form RNA with single strand DNA hybridization probes generated by asymmetric PCR

不对称PCR制备单链探针检测登革Ⅱ型病毒复制型RNA和复制中间体RNA

Results Continuing PAGE showed the specific single sequence of the two target products could be amplified by asymmetric PCR , and the size was accord with expectation .

结果：连续胶PAGE表明非对称PCR可分别扩增出两个靶序列的特异性单链，大小符合预期。

By using RAPD primer mediated asymmetric PCR ( RM-PCR ) method , a newtype of molecular marker based on the telomeric repeats sequence was developed .

利用RAPD引物介寻的不对称复性温度PCR的方法（RM－PCR），发展了一种基于端粒重复序列的新型分子标记。

Results By asymmetric PCR , half of shadow bands of dinucleotide repeats were eliminated and there was only one band for each allele for tetranucleotide sequence .

结果利用不对称PCR，某些二核苷酸（CA）n重复序列的影子带减少一半，四核苷酸重复序列的影子带消除；

Conclusion The difference between sense and antisense strands in electrophoresis is one of the causes in shadow bands , and these shadow bands can be eliminated by asymmetric PCR partly or totally .

结论正负链电泳行为的差异是影子带产生的原因之一，利用不对称PCR可部分或完全消除影子带。

By means of asymmetric PCR and indirect fluorescent-labeling and technology to amplify and label the samples , they were hybridized with the oligonucleotide microarrays , followed by washing , scanning and analysis .

采用不对称PCR和间接荧光标记技术进行单链DNA扩增和荧光标记，标记样品与寡核苷酸芯片杂交后，进行芯片清洗、扫描及结果分析。

Methods Asymmetric PCR based method was utilized to synthesize the 1 202 bp 3D7 / msp1 42 gene . The expressing plasmid containing the synthetic gene was introduced into Pichia pastoris by electroporation .

方法采用不对称PCR法拼接合成了3D7/msp142基因（1202bp），用电穿孔法将合成基因导入毕赤酵母并进行诱导表达，用免疫印迹法检测表达产物。

To select suitable reaction condition of asymmetric PCR in the synthesis of insulin containing specific mutation points , further research on the temperature to allow the primers to anneal and the varied proportions to different primers between the mutation and the normal terminal was carried out .

在胰岛素定点突变体合成中，对不对称PCR反应中突变位点的引物和末端引物的比例以及退火温度进行优化。

Meanwhile , Using TAIL-PCR ( Thermal Asymmetric Interlace PCR ) technology obtained and analyzed Transferred-DNA flanking sequences .

运用TAIL-PCR（热不对称交错PCR）技术获取和分析T-DNA侧翼序列。

This was the first time to clone flavonoid 3 ' - hydroxy-lase gene from genomic DNA of woody plants by thermal asymmetric interlaced PCR .

这是首次用热不对称交错PCR法从木本植物的基因组DNA克隆到类黄酮3'-羟化酶基因。

A T-DNA tagged rice population was generated for functional genomic analysis . Using thermal asymmetric interlaced PCR ( TAIL-PCR ), 159 sequences flanking the right border of T-DNA were obtained .

利用热不对称交错PCR（TAILPCR），对200个含TDNA插入的转基因水稻株系进行分析，获得了159个TDNA右边界侧翼序列。

According to the cDNA sequences data , the first gene sequences encoding the amphibian bradykinin were successfully obtained by using the total DNA from O. grahami as template , and by means of methods such as thermal asymmetric interlaced PCR ( TAIL-PCR ) .

根据这些cDNA序列，以无指盘臭蛙皮肤总DNA为模板，利用热不对称交错PCR（TAIL-PCR）等方法，本研究还成功获得了首条两栖动物缓激肽基因序列。